Objective to investigate the effects of FEZ family zinc finger 1-antisense RNA 1(LncRNA FEZF1-AS1) on the proliferation, invasion and epithelial interstitial transition (EMT) of pulmonary fibrosis cells and its mechanism. Methods Transforming growth factor β1(TGF-β1) was applied to A549 cells to induce pulmonary interstitial fibrosis cell models, which were divided into A549 cells group (blank group) and model group. The protein expression levels of E-cadherin, N-cadherin and Vimentin in each group were detected by western blotting method to observe whether the modeling was successful. The expressions of LncRNA FEZF1-AS1 and miR-200c-3p in the two groups were detected by Quantitative Real-time PCR (qRT-PCR). According to the experimental purpose and transfection plasmid, the cells were divided into blank group, TGF-β1+Si LncRNA FEZF1-AS1 NC group, TGF-β1+Si LncRNA FEZF1-AS1 group. Cell proliferation ability was detected by CCK-8 method, cell migration ability was detected by cell scratch, and cell invasion ability was detected by Transwell assays. The protein expressions levels of E-cadherin, N-cadherin and Vimentin in each group were detected by western blotting. LncRNA FEZF1-AS1 and miR-200c-3p gene expression levels in each group were detected by qRT-PCR. Results Compared with the blank group, the protein expression level of E-cadherin in the modeling group was decreased (P < 0.05)，and the protein expression levels of N-cadherin and Vimentin were increased (P < 0.05). Compared with the blank group, the expression level of LncRNA FEZF1-AS1 gene in the modeling group was increased (P < 0.05), and the expression level of miR-200c-3p gene was decreased (P < 0.05). Compared with blank group, the proliferation, migration and invasion ability of TGF-β1+Si LncRNA FEZF1-AS1 NC group were higher (P < 0.05). Compared with TGF-β1+Si LncRNA FEZF1-AS1 NC group, the cell proliferation, invasion and migration ability of TGF-β1+Si LncRNA FEZF1-AS1 group were decreased (P < 0.05). Compared with TGF-β1+Si LncRNA FEZF1-AS1 NC group, LncRNA FEZF1-AS1 gene expression and protein expressions of N-cadherin and Vimentin were decreased in TGF-β1+Si LncRNA FEZF1-AS1 group, and the expression of E-cadherin protein was increased. Compared with TGF-β1+Si LncRNA FEZF1-AS1 NC group, the expression of miR-200c-3p gene in TGF-β1+Si LncRNA FEZF1-AS1 and blank groups were increased (P < 0.05). Conclusions LncRNA FEZF1-AS1 can promote the proliferation, invasion, metastasis and EMT process of pulmonary interstitial fibrosis cells by inhibiting miR-200c-3p.