为了研究黄花倒水莲花中黄酮类成分及其抗氧化活性。本实验采用硅胶、Sephadex LH-20柱色谱和HPLC进行分离纯化,并结合1D-NMR波谱数据和文献比对,从黄花倒水莲花95%乙醇提取物的正丁醇部位中分离鉴定出6个黄酮苷类化合物：紫云英苷(1),槲皮素-3-O-β-D-葡萄糖苷(2),槲皮素-3-O-β-吡喃木糖苷(3),槲皮素-3-O-α-L-吡喃阿拉伯糖苷(4),异鼠李素-3-O-β-葡萄糖苷(5),芦丁(6),化合物1-6均为首次从该植物中分离得到；采用FRAP法对所获得化合物进行总抗氧化能力评价,结果表明化合物1-6均显示出较好的抗氧化作用,其中化合物4的总抗氧化能力达到4.533 mmol/g(Vc为5.951 mmol/g)。研究结果不仅丰富黄花倒水莲花的物质基础,而且为其今后的开发利用提供科学依据。
To study the chemical constituents and antioxidant activity of the flavones in flowers from thePolygala fallaxHemsl. Six compounds were isolatedand purified by silica gelcolumn chromatography, Sephadex LH-20 and preparative high performance liquid chromatography (HPLC)from the n-butanolfraction of 95% ethanol extract of Polygala fallax. Combined with the 1D-NMR spectral data and literature comparison, their structures were identified as kaempferol-3-glucoside (1), quercetin-3-O-β-D-galactoside (2), quercetin-3-O-β-xylopyranoside (3), quercetin-3-O-α-L-arabinoside (4), isorhamnetin-3-O-β-D-glucopyranoside (5), rutin (6), respectively. Compounds 1-6 are obtained from the plant for the first time.The antioxidant activities of compounds were evaluated by using FRAP methods. The results showed that all compounds had better antioxidant capacity.Among of them, total antioxidant activity of compound 4 was up to 4.533 mmol/g(vitamin C was of 5.951 mmol/g).Thus, the overall results not only enrich the material basis of flowers of Polygala fallax Hemsl, but also provide scientific basis for its future development and utilization.